RHAMM Antibody

RHAMM Antibody: The hyaluronan-mediated motility receptor, also known as RHAMM, was initially identified as a soluble protein that could be released by sub-confluent migrating cells, promoting cell motility and invasion via interactions with hyaluronan (HA) and the cell surface. While RHAMM is normally poorly expressed in most normal tissues and is not required for embryonic development or normal cell homeostasis functions, its expression is increased during wound repair in response to hypoxia and fibrogenic factors. However, its overexpression is transforming in multiple types of cancers and is required for maintaining RAS transformation. RHAMM associates with BRCA1 and BARD1, attenuating the mitotic-spindle-promoting activity of RHAMM, which may contribute to tumor progression by promoting genomic instability.

KO Validation (Figure 1) : Anti-RHAMM antibodies (6189) specificity was further verified by RHAMM specific knockout. RHAMM signal was not detected in RHAMM knockout HeLa cells as compared to that in control wild type cells. KO Validation

RHAMM antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Hardwick C, Hoare K, Owens R, et al. Molecular cloning of a novel hyaluronan receptor that promotes tumor cell motility. J. Cell Biol. 1992; 117:1343-50.

Samuel SK, Hurta RA, Spearman MA, et al. TGF-beta 1 stimulation of cell locomotion utilizes the hyaluronan receptor RHAMM and hyaluronan. J. Cell Biol. 1993; 123:749-58.

Hall CL, Yang B, Yang X, et al. Overexpression of the hyaluronan receptor RHAMM is transforming and is also required for H-ras transformation. Cell 1995; 82:19-26.