ZBTB5 Antibody

ZBTB5 Antibody: The ZBTB family of proteins is comprised of diverse zinc finger proteins that also contain a BTB (BR-C, ttk and bab) domain. ZBTB5 was identified though sequence analysis as a POZ domain Kruppel-like zinc finger (POK) protein. Further experiments indicated that it binds DNA and can directly repress transcription of the cell cycle arrest gene p21. ZBTB5 can also interact with co-repressor histone deacetylase complexes such as BCoR, NCoR, and SMRT via its POZ domain, resulting in deacetylation of histones Ac-H3 and Ac-H4 at the proximal promoter. ZBTB5 stimulates both cell proliferation and cell cycle progression, suggesting that it may act as a potential proto-oncogene.

ZBTB5 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

At least two isoforms of ZBTB5 are known to exist; this antibody will recognize both isoforms. This antibody is predicted to not cross-react with other ZBTB protein family members.

Strausberg RL, Feingold EA, Grouse LH, et al. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc. Natl. Acad. Sci. USA2002; 99:16899-903.

Koh D, Choi W, Jeon B, et al. A novel POK family transcription factor, ZBTB5, represses transcription of p21CIP1 gene. J. Biol. Chem.2009; 284:19856-66.