KPNA2 Antibody

Product Specifications

Background

KPNA2 Antibody: Karyopherin, a cytosolic and heterodimeric protein complex consisting of alpha and beta subunits, is responsible for targeting proteins with nuclear localization signals to the nuclear pore complex by an energy requiring, Ran-dependent mechanism. The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC. KPNA2 is the alpha subunit 2 of karyopherin, which forms a complex with importin subunit beta-1 and functions as a cargo carrier that transports various complexes from cytoplasm into nucleus. It is ubiquitously expressed and contains an IBB/importin beta domain, ten Armadillo repeats that bind "cargo" and three intervening nuclear localization sequences (NLSs) . It has recently been reported to play an important role in tumorigenesis and tumor progression.

NCBI Gene ID

3838

Swiss Prot

P52292

Accession Number

NP_002257

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer, Cell Cycle

Purification

KPNA2 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1461 - Rat Heart Tissue Lysate

Concentration

1 mg/mL

Buffer

KPNA2 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

KPNA2 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Symbol

KPNA2

Positive Control 2

Cat. No. 10-501 - Human Heart Tissue Slide

Positive Control 3

Cat. No. 1224 – Daudi Cell Lysate

NCBI Official Name

Karyopherin alpha 2 (RAG cohort 1, importin alpha 1)

NCBI Organism

Homo sapiens

Background Reference 01

Moroianu J. Molecular mechanisms of nuclear protein transport. Crit. Rev. Eukaryot. Gene Expr.1997; 7:61-72.

Background Reference 02

Gilchrist D and Rexach M. Molecular basis for the rapid dissociation of nuclear localization signals from karyopherin alpha in the nucleoplasm. J. Biol. Chem.2003; 278: 51937-49.

Background Reference 03

Tseng SF, Chang CY, Wu KJ, et al. Importin KPNA2 is required for proper nuclear localization and multiple functions of NBS1. J. Biol. Chem.2005; 280:39594-600.

Background Reference 04

Sakai M, Sohda M, Miyazaki T, et al. Significance of karyopherin-{alpha} 2 (KPNA2) expression in esophageal squamous cell carcinoma. Anticancer Res.2010; 30:851-6.