CRISP2 Antibody

CRISP2 Antibody: The cysteine-rich secretory proteins (CRISP) family is a group of four proteins that are strongly expressed in the male reproductive tract and have been implicated in having roles in male fertility. CRISP2, also known as TPX1, has been implicated in the adhesion between spermatids and Sertoli cells, and with CRISP1, is thought to be involved in sperm-egg fusion. CRISP2 has been shown to regulate the Ca2+ influx through ryanodine receptors (RYR) and may influence the acrosome reaction or sperm motility. CRISP2 has also been shown to bind to the mitogen-activated protein kinase kinase kinase 11 (MAP3K11) and localizes to the developing acrosome, suggesting this CRISP2-MAP3K11 complex may have a role in acrosome development.

CRISP2 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cohen DJ, Busso D, Da Ros V, et al. Participation of cysteine-rich secretory proteins (CRISP) in mammalian sperm-egg interaction. Int. J. Dev. Biol.2008; 52:737-42.

Maeda T, Sakashita M, Ohba Y, et al. Molecular cloning of the rat Tpx-1 responsible for the interaction between spermatogenic and Sertoli cells. Biochem. Biophys. Res. Commun.1998; 248:140-6.

Busso D, Goldweic NM, Hayashi M, et al. Evidence for the involvement of testicular protein CRISP2 in mouse sperm-egg fusion. Biol. Reprod.2007; 76:701-8.

Gibbs GM, Scanlon MJ, Swarbrick J, et al. The cysteine-rich secretory protein domain of Tpx-1 is related to ion channel toxins and regulates ryanodine receptor Ca2+ signaling. J. Biol. Chem.2006; 281:4156-63.