PIAS4 Antibody

Product Specifications

Background

PIAS4 Antibody: The PIAS (protein inhibitor of activated STAT) proteins play a crucial role as transcriptional coregulators in various cellular pathways, including the STAT, p53 and the steroid hormone signaling pathway. The PIAS protein family includes at least five evolutionarily conserved genes, including PIAS4. The major function of the PIAS proteins is the control of gene transcription and can also act as small ubiquitin-like-modifier (SUMO) E3 ligases. PIAS4 interacts with p53 and blocks its ability to induce Bax and p21 transcription. PIAS4 is also important in the control of the ubiquitin-dependent proteasomal degradation of the Ets-1 transcription factor. PIAS4 has been implicated in the DNA-damage response pathway and is thought to work in combination with PIAS1 for the productive association of 53BP1, BRCA1 and RNF168.

NCBI Gene ID

51588

Swiss Prot

Q8N2W9

Accession Number

NP_056981

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Signal Transduction

Purification

PIAS4 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1209 - HL60 Cell Lysate

Concentration

1 mg/mL

Buffer

PIAS4 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

PIAS4 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Symbol

PIAS4

NCBI Official Name

Protein inhibitor of activated STAT, 4

NCBI Organism

Homo sapiens

Background Reference 01

Shuai K and Liu B. Regulation of gene-activation pathways by PIAS proteins in the immune system. Nat. Rev. Immunol. 2005; 5:593-605.

Background Reference 02

Nelson V, Davis GE, and Maxwell SA. A putative protein inhibitor of activated STAT (PIASy) interacts with p53 and inhibits p53-, ediated transactivation but not apoptosis. Apoptosis 2001; 6:221-34.

Background Reference 03

Nishida T, Terashima M, Fukami K, et al. PIASy controls ubiquitin-dependent proteasomal degradation of Ets-1. Biochem. J. 2007; 405:481-8.

Background Reference 04

Galanty Y, Belotserkovskaya R, Coates J, et al. Mammalian SUMO E3-ligases PIAS1 and PIAS4 promote responses to DNA double-strand breaks. Nature 2009; 462:935-9.