KLF4 Antibody

KLF4 Antibody: KLF4 is a transcription factor that functions as both a transcriptional activator and repressor to regulate proliferation and differentiation of multiple cell types. The role of KLF4 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing KLF4 and the transcription factors POU5F1, Sox2, and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing KLF4, Sox2, POU5F1 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. KLF4 interacts directly with POU5F1 and Sox2 in iPS and ES cells and activates the target gene NANOG.

Relative Expression Validation (Figure 1) : Anti-KLF4 antibodies (5603) specificity was further verified by KLF4 relative expression. KLF4 protein was detected in MCF-7 cells, but not in Jurkat cells, which matches the RNA expression pattern in these two cell lines based on Human Protein Atlas data (https://www.proteinatlas.org/ENSG00000136826-KLF4/subcellular) . Relative Expression Validation5603) KLF4 protein was detected in MCF-7 cells, but not in Jurkat cells, which matches the RNA expression pattern in these two cell lines based on Human Protein Atlas data (https://www.proteinatlas.org/ENSG00000136826-KLF4/subcellular

KLF4 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Human KLF4 has 5 isoforms including isoform 1 (513aa, 55kD), isoform 2 (479aa, 51kDa), isoform 3 (429aa, 46kDa), isoform 4 (118aa, 12kDa), and isoform 5 (63aa, 6.5kDa) ; Mouse KLF4 has one isoform (483aa, 52kD and rat KLF4 has one isoform (482aa, 52kD) .

Evans PM, Zhang W, Chen X, et al. Kruppel-like factor 4 is acetylated by p300 and regulates gene transcription via modulation of histone acetylation. J. Bio. Chem.2007; 282:33994-4002.

Takahashi K and Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell2006; 1263:663-76.