NANOG Antibody

Product Specifications

Background

NANOG Antibody: Expression of NANOG is required for the maintenance of pluripotency in epiblast and embryonic stem (ES) cells as well as for the ability to maintain ES self-renewal independently of LIF/Stat3. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.

NCBI Gene ID

79923

Swiss Prot

Q9H9S0

Accession Number

EAW88651

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Homeostasis, Stem Cell

Purification

NANOG Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1416 – Mouse Testis Tissue Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Bovine: (89%), Chicken: (94%)

Buffer

NANOG Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

NANOG antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

Predicted: 35 kD Observed: 35 kD

Fragment

IgG

Isoform

Human NANOG has 2 isoforms, isoform 1 (305aa, 34.6kD) and isoform 2 (289aa, 32.8kDa) . Mouse NANOG has 2 isoforms, isoform 1 (305aa, 34.2kD) and isoform 2 (280aa, 31.9kD) . Rat NANOG has 1 isoform (311aa, 35.1kD) . 5585 can detect NANOG in human, mouse, and rat.

Symbol

NANOG

Positive Control 2

Cat. No. 1476 – Rat Testis Tissue Lysate

NCBI Official Name

Nanog homeobox

NCBI Organism

Homo sapiens

Background Reference 01

Chambers I, Colby D, Robertson M, et al. Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells. Cell2003; 113:643-55.

Background Reference 02

Mitsui K, Tokuzowa Y, Itoh H, et al. The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells. Cell2003; 113:631-42.