SQSTM1 Antibody

Product Specifications

Background

SQSTM1 Antibody: SQSTM1/p62 is an adapter protein which binds ubiquitin and regulates signaling cascades through ubiquitination. It may regulate the activation of NF-κ B by TNF-α , nerve growth factor (NGF) and interleukin-1. SQSTM1/p62, a co-interacting protein of the atypical PKC isoforms, has a UBA domain at its C-terminal end, which binds non-covalently to polyubiquitin chain. SQSTM1's UBA domain is necessary for recruitment of polyubiquitin and aggresome formation. SQSTM1 may play a role in titin/TTN downstream signaling in muscle cells and may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels. Mutations in the ubiquitin-associated (UBA) domain of SQSTM1 commonly cause Paget's disease of bone since the UBA is necessary for aggregate sequestration and cell survival.

NCBI Gene ID

8878

Swiss Prot

Q13501

Accession Number

Q13501

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Validation

KO Validation (Figure 1) : Anti-SQSTM1 antibodies (5449) specificity was further verified by SQSTM1 specific knockout. SQSTM1 signal was not detected in SQSTM1 knockout HEK293T cells as compared to that in control wild type cells. KO Validation5449) Induced Expression Validation (Figure 2) : Raw 264.7 cells were treated with LPS (0.3 µg /mL) for different time period (0-24 hrs) . SQSTM1 expression detected by anit-SQSTM1 antibodies (5449) was induced by LPS treatment and the expression levels were increased gradually over the period of 24 hrs. Induced Expression Validation5449

Field of Research

Homeostasis

Purification

SQSTM1 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1306 - Human Spleen Tissue Lysate

Concentration

1 mg/mL

Buffer

SQSTM1 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

SQSTM1 antibody can be stored at 4˚ C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Isoform

Human SQSTM1 has two isoforms, including isoform 1 (440aa, 47.7kD) and isoform 2 (356aa, 38.6kD) . Mouse SQSTM1 also has two isoforms, including isoform 1 (442aa, 48.2kD) and isoform 2 (404aa, 44.2kD) . Rat SQSTM1 has three isoforms, including isoform 1 (439aa, 47.7kD), isoform 2 (412aa, 45kD) and isoform 3 (234aa, 26.1kD) . 5449 can detect the isoforms of human mouse and rat except the shortest rat isoform 3.

Symbol

SQSTM1

Positive Control 2

Cat. No. 1204 - K562 Cell Lysate

Positive Control 3

Cat. No. 1201 – HeLa Cell Lysate

Positive Control 4

Cat. No. 1203 – A549 Cell Lysate

Positive Control 5

Cat. No. 1282 – 3T3/NIH Cell Lysate

NCBI Official Name

Sequestosome 1

NCBI Organism

Homo sapiens

Background Reference 01

Seibenhener et al. Mol. Cell. Biol.2004; 24:8055-68.

Background Reference 02

Hocking et al. Hum. Mol. Genet.2002; 11:2735-9.

Background Reference 03

Rousiere et al. Best Pract. Res. Clin. Rheumatol.2003; 17:1019-41.

Background Reference 04

Layfield et al. Biochem. Soc. Trans.2004; 32:728-30.

Other Product Names

SQSTM1 Antibody: p60, p62, A170, OSIL, PDB3, ZIP3, p62B, ORCA, Sequestosome-1, EBI3-associated protein of 60 kDa, EBIAP

Tested Applications

ELISA, WB, IHC-P, IF

Protein ID

74735628

Physical Properties

Liquid

Available Sizes

Curated Selection

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