GOLPH1 Antibody
Product Specifications
Background
GOLPH1 Antibody: GOLPH1, also known as GCP60, was initially identified as a Golgi protein that can interact with the integral membrane protein giantin and is thought to be involved in the maintenance of the Golgi structure. GOLPH1 has also been shown to interact with other Golgi proteins such as Golgin-160, a Golgi protein that can be cleaved by caspases-2, -3, and -7, leading to the nuclear localization of Golgin-160. GOLPH1 interaction with the Golgin-160 fragments is stronger than that with the intact Golgin-160, with its interaction regulated by the oxidation state of Cys-463 within GOLPH1, suggesting that the nuclear localization of the caspase-cleaved Golgin-160 fragments is a highly coordinated event. GOLPH1 has also been found to interact with Numb, a cytosolic signaling protein that mediates asymetric cell division of neural progenitor cells to a daughter progenitor cell and a neuron, suggesting that Golgi fragmentation and reconstitution during the cell cycle differentially regulate Numb signaling through changes in GOLPH1 subcellular distribution and may couple cell fate with cell cycle progression.
NCBI Gene ID
64746
Swiss Prot
Q9H3P7
Accession Number
EAW69775
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Stem Cell
Purification
GOLPH1 Antibody is affinity chromatography purified via peptide column.
Positive Control
Cat. No. 1204 - K562 Cell Lysate
Concentration
1 mg/mL
Homology
Predicted species reactivity based on immunogen sequence: Mouse: (83%), Rat: (83%)
Buffer
GOLPH1 Antibody is supplied in PBS containing 0.02% sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
GOLPH1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=5439
Fragment
IgG
Symbol
ACBD3
NCBI Official Name
Acyl-Coenzyme A binding domain containing 3
NCBI Organism
Homo sapiens
Background Reference 01
Sohda M, Misumi Y, Yamamoto A, et al. Identification and characterization of a novel Golgi protein, GCP60, that interacts with the integral membrane protein giantin. J. Biol. Chem.2001; 276:45298-306.
Background Reference 02
Mancini M, Machamer CE, Roy S, et al. Caspase-2 is localized at the Golgi complex and cleaves golgin-160 during apoptosis. J. Cell Biol.2000; 149:603-12.
Background Reference 03
Sbodio JI and Machamer CE. Identification of a redox-sensitive cysteine in GCP60 that regulates its interaction with Golgin-160. J. Biol. Chem.2007; 282:29874-81.
Background Reference 04
Zhou Y, Atkins JB, Rompani SB, et al. The mammalian Golgi regulates numb signaling in asymetric cell division by releasing ACBD3 during mitosis. Cell2007; 129:163-78.
Other Product Names
GOLPH1 Antibody: PAP7, GCP60, GOCAP1, GOLPH1, Golgi resident protein GCP60, Acyl-CoA-binding domain-containing protein 3
Tested Applications
ELISA, WB
Protein ID
119590181
Physical Properties
Liquid
Available Sizes
Curated Selection
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