IRGC Antibody

Product Specifications

Background

IRGC Antibody: Immunity-related GTPases (IRG) (also known as p47 GTPases) are a family of GTPase proteins found in vertebrates, which play critical roles in mediating innate resistance to intracellular pathogens. IRG genes have been found in a number of mammals and lower species including mice, rats, zebrafish and humans. Most of the mouse genes contain interferon-stimulated response elements which mediate transcriptional activation by IFNs. In humans, only two IRG genes have been found: human IRGC encodes a full-length IRG protein that, like the mouse homologue, is constitutively expressed in testis, while human IRGM encodes a considerably truncated protein that is constitutively expressed in cultured cells including some macrophage cell lines. As the two human genes IRGC and IRGM are not subject to IFN control, it has been suggested that the host resistance mechanism supported by IRG proteins in the mouse is lacking in humans.

NCBI Gene ID

56269

Swiss Prot

Q6NXR0

Accession Number

NP_062558

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Innate Immunity

Purification

IRGC Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1403 - Mouse Brain Tissue Lysate

Concentration

1 mg/mL

Buffer

IRGC Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

IRGC antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Specificity

Two isoforms of IRGC are known to exist; this antibody will recognize both isoforms.

Symbol

IRGC

Positive Control 2

Cat. No. 17-208 - A-20 Cell Slide

NCBI Official Name

Immunity-related GTPase family, cinema

NCBI Organism

Homo sapiens

Background Reference 01

Taylor GA. IRG proteins: key mediators of interferon-regulated host resistance to intracellular pathogens. Cell Microbiol.2007; 9:1099-107.

Background Reference 02

Yamada K, Akimoto H, Ogawa Y, et al. Upregulation of immunity-related GTPase (IRG) proteins by TNF-alpha in murine astrocytes. Biochem. Biophys. Res. Commun.2009; 382:434-9.

Background Reference 03

Singh SB, et al. Human IRGM induces autophagy to eliminate intracellular mycobacteria. Science2006; 313:1438-41.

Background Reference 04

Bekpen C, Hunn JP, Rohde C, et al. The interferon-inducible p47 (IRG) GTPases in vertebrates: loss of the cell autonomous resistance mechanism in the human lineage. Genome Biol.2005; 6:R92.