NOTUM Antibody

Product Specifications

Background

NOTUM Antibody: In Drosophila, the notum gene is regulated by the Wingless pathway and encodes a secreted hydrolase that modifies heparan sulfate proteoglycans. The mammalian homolog has been shown to be able to cleave glypicans and can release GPI-anchored proteins from the mammalian cell surface. Like the Drosophilia NOTUM, the mammalian protein can act as a negative regulator of the Wnt signaling pathway. NOTUM is expressed at a low level in most mammalian tissues, although it is overexpressed in a subset of human hepatocellular carcinomas. Its transcription is regulated by beta-catenin/TCF and is a target of the Wnt signaling pathway, forming a negative feedback loop that regulates the expression and activity of the Wnt pathway.

NCBI Gene ID

147111

Swiss Prot

Q6P988

Accession Number

NP_848588

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Signal Transduction

Purification

NOTUM Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1305 - Human Kidney Tissue Lysate

Concentration

1 mg/mL

Buffer

NOTUM Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

NOTUM antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Symbol

NOTUM

Positive Control 2

Cat. No. 10-301 - Human Brain Tissue Slide

NCBI Official Name

Notum pectinacetylesterase homolog (Drosophila)

NCBI Organism

Homo sapiens

Background Reference 01

Giraldez AJ, Copley RR, and Cohen SM. HSPG modification by the secreted enzyme Notum shapes the wingless morphogen gradient. Dev. Cell2002; 667-76.

Background Reference 02

Traister A, Shi W, and Filmus J. Mammalian Notum induces the release of glypicans and other GPI-anchored proteins from the cell surface. Biochem. J.2008; 410:503-11.

Background Reference 03

Torisu Y, Watanabe A, Nonaka A, et al. Human homolog of NOTUM, overexpressed in hepatocellular carcinoma, is regulated transcriptionally by b-catenin/TCF. Cancer Sci.2008; 6:1139-46.