MPYS Antibody

Product Specifications

Background

MPYS Antibody: MPYS is a recently identified plasma membrane tetraspanner that is associated with major histocompatibility complex class II (MHC-II) and mediates its transduction of apoptotic signals. It has also been found to be associated with VISA, a mitochondrial protein that acts as an adaptor in virus-triggered signaling. MPYS also interacts with IRF3 and recruits the kinase TBK1 to the VISA-associated complex, acting as a critical mediator of virus-triggered IRF3 activation and interferon (IFN) expression. It is thought that the binding of nucleic acid to the innate immune protein RIG-I causes complex formation between RIG-I, VISA, and MPYS. This complex then recruits TBK1 to phosphorylate IRF3 which then directly activates IFN transcription. At least three isoforms of MPYS are known to exist.

NCBI Gene ID

340061

Swiss Prot

Q86WV6

Accession Number

NP_938023

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

5269

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Validation

Independent Antibody Validation in Cell lines (Figure 1) shows similar MPYS expression profile in human cell lines detected by two independent anti-MPYS antibodies that recognize different epitopes, 5269 against near C-terminus domain and 64-236 against C-terminus domain. MPYS proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines5269 64-236

Field of Research

Innate Immunity

Purification

MPYS Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1204 - K562 Cell Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Rat (82%), Bovine: (82%), Pig: (82%), Chicken: (71%)

Buffer

MPYS Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

MPYS antibody can be stored at 4˚ C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Isoform

Human MPYS has one isoform (379aa, 42kD) . Mouse MPYS has 3 isoforms, including isoform 1 (379aa, 42kD), isoform 2 (427aa, 48kD) and isoform 3 (337aa, 38kD) . Rat MPYS also has one isoform (379aa, 43kD) . 5269 can detect human, mouse and rat.