CIP75 Antibody

CIP75 Antibody: The ubiquitin-proteosome pathway of protein degradation is one of the mechanisms that ensure the proper level of cellular proteins. The ubiquitin-like (UbL) and ubiquitin-associated (UBA) domain containing protein family is thought to be involved in proteosomal degradation. One such protein is CIP75, also known as ubiquilin-4, interacts with a number of proteins such as Ataxin-1 and Connexin-43, resulting in an increased rate of turnover of these proteins. Overexpression CIP75 led to a significant reduction of Connexin-43 half-life, with the opposite being observed in siRNA knockdown experiments. CIP75 contains an N-terminal UbL domain which is thought to interact with proteins of the 26S proteosome complex and a C-terminal UBA domain which appears to mediate its interaction with Connexin-75. At least three isoforms of CIP75 are known to exist.

CIP75 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Su V and Lau AF. Ubiquitin-like and ubiquitin-associated domain proteins: significance in proteasomal degradation. Cell. Mol. Life Sci.2009; 66:2819-33.

Davidson JD, Riley B, Burright EN, et al. Identification and characterization of an ataxin-1-interacting protein: A1Up, a ubiquitin-like protein. Hum. Mol. Genet.2000; 9:2305-12.

Li X, Su V, Kurata WE, et al. A novel Connexin43-interacting protein, CIP75, which belongs to the UbL-UBA protein family, regulates the turnover of Connexin4 J. Biol. Chem.2008; 283:5748-59.