THEM2 Antibody

Product Specifications

Background

THEM2 Antibody: THEM2, also known as ACOT13, belongs to the hotdog-fold superfamily and possesses thioesterase activity, with greater activity observed with longer chain acyl-CoAs such as myristoyl- and palmitoyl-CoA. THEM2 is highly expressed in several tissues such as heart, kidney, liver and brain and has been shown to be co-localized with beta-tubulin on microtubules. THEM2 interacts with StarD, a protein that plays a key role in fatty acid metabolism, and the addition of StarD to THEM2 increased its catalytic activity, suggesting that THEM2 plays a significant role in the metabolism of fatty acids. At least two isoforms of THEM2 are known to exist.

NCBI Gene ID

55856

Swiss Prot

Q9NPJ3

Accession Number

NP_060943

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Obesity

Purification

THEM2 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1211 - HepG2 Cell Lysate

Concentration

1 mg/mL

Buffer

THEM2 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

THEM2 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Symbol

ACOT13

Positive Control 2

Cat. No. 10-201 - Human Liver Tissue Slide

NCBI Official Name

Acyl-CoA thioesterase 13

NCBI Organism

Homo sapiens

Background Reference 01

Cheng Z, Song F, Shan X, et al. Crystal structure of human thioesterase superfamily member 2. Biochem. Biophys. Res. Commun.2006; 349:172-7.

Background Reference 02

Wei J, Kang HW, and Cohen DE. Thioesterase superfamily member 2 (Them2) /acyl-CoA thioesterase 13 (Acot13) : a homotetrameric hotdog fold thioesterase with selectivity for long-chain fatty acyl-CoAs. Biochem. J.2009; 421:311-22.

Background Reference 03

Cheng Z, Bao S, Shan X, et al. Human thioesterase superfamily member 2 (hTHEM2) is co-localized with beta-tubulin onto the microtubule. Biochem. Biophys. Res. Commun.2006; 350:850-3.

Background Reference 04

Scappa EF, Pocai A, Wu MK, et al. Regulation of energy substrate utilization and hepatic insulin sensitivity by phosphatidylcholine transfer protein/StarD. FASEB J.2008; 22:2579-90.