SYPL2 Antibody

Product Specifications

Background

SYPL2 Antibody: SYPL2, also known as Mitsugumin 29, was initially identified as a transmembrane protein from the triad junction in skeletal muscle that had significant homology with members of the synaptophysin family. SYPL2 is thought to participate in the excitation-contraction coupling process of skeletal muscle as SYPL2-null mice showed reduced muscle contractile force and altered triad junction structure and increased susceptibility to fatigue of the skeletal muscle. SYPL2 plays a critical role in muscle Ca2+ signaling by regulating the process of store-operated Ca2+ entry and interacts with ryanodine receptor (RyR), thereby influencing intracellular Ca2+ homeostasis through changes in the RyR/Ca2+ release function. Co-expression of SYPL2 and RyR in cultured cells leads to apoptotic cell death resulting from the depletion of Ca2+ from the intracellular stores.

NCBI Gene ID

284612

Swiss Prot

Q5VXT5

Accession Number

NP_001035799

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Signal Transduction

Purification

SYPL2 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1306 - Human Spleen Tissue Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Rabbit: (100%)

Buffer

SYPL2 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

SYPL2 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Specificity

At least two isoforms of SYPL2 are known to exist. SYPL2 antibody will not cross-react with SYPL1.

Symbol

SYPL2

Positive Control 2

Cat. No. 10-901 - Human Spleen Tissue Slide

NCBI Official Name

Synaptophysin-like 2

NCBI Organism

Homo sapiens

Background Reference 01

Takeshima H, Simuta M, Komazaki S, et al. Mitsugumin29, a novel synaptophysin family member from the triad junction in skeletal muscle. Biochem. J.1998; 331:317-22.

Background Reference 02

Nishi M, Komazaki S, Kurebayashi Y, et al. Abnormal features in skeletal muscle from mice lacking mitsugumin29. J. Cell Biol.1999; 147:1473-80.

Background Reference 03

Nagaraj RY, Nosek CM, Brotto MA, et al. Increased susceptibility to fatigue of slow- and fast-twitch muscles from mice lacking the MG29 gene. Physiol. Genom.2000; 4:43-9.