CLDN1 Antibody

CLDN1 Antibody: Claudin1 (CLDN1), a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Tight junctions are specialized regions of cell to cell contact consisting of networking strands that act as a molecular gasket for preventing the leakage of ions, water, etc., between cells. They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different Claudin composition and CLDN1 expression is often cell type and tissue dependent. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome. CLDN1 and CLDN2 were found to be overexpressed in colonal cancer tissues and may be useful as tumor markers and targets for the treatment of colorectal cancer. Characterization of Claudins expression in human tumors can be an additional diagnostic tool. Recent studies show that CLDN1 has gastric tumor suppressive activity and is a direct transcriptional target of RUNX3. Along with SCARB1, LDL-R, and the tetraspanin superfamily member CD81, CLDN1 has been reported to be an entry factor for the Hepatitis C virus.

Independent Antibody Validation in Cell lines (Figure 1) shows similar CLDN1 expression profile in human cell lines detected by two independent anti-CLDN1 antibodies that recognize different epitopes, 5187 against C-terminus domain and 24-027 against recombinant fusion protein. CLDN1 proteins are detected in some tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines5187 24-027

CLDN1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Human CLDN1 has one isoform (211aa, 23kD) . Mouse CLDN1 has one isoform (211aa, 23kD) and Rat CLDN1 also has one isoform (211aa, 23kD) . 5187 can detect human, mouse and rat.