RSPO1 Antibody

Product Specifications

Background

RSPO1 Antibody: RSPO1 is a member of a family of secreted growth factors that can operate through the canonical Wnt signaling pathway by stabilizing the intracellular beta-catenin, thereby regulating functions mediated by beta-catenin such as cell fate decisions and embryonic patterning. RSPO1 was recently identified through linkage analysis to be involved in sex determination and mammalian ovarian development. RSPO1 is thought to regulate cellular responsiveness to Wnt ligands by modulating the cell-surface expression of the Wnt co-receptor LRP6 by interfering with the DKK/Kremen-mediated internalization of LRP6 through an interaction with Kremen, resulting in increased LRP6 cell-surface levels.

NCBI Gene ID

284654

Swiss Prot

Q2MKA7

Accession Number

NP_001033722

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

5171

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Validation

Recombinant Protein Test (Figure 2) : Anti-RSPO1 antibodies (5171) detected human RSPO1 recombinant protein at different concentrations. Recombinant Protein Test5171

Field of Research

Signal Transduction

Purification

RSPO1 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1224-Daudi Cell Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Rat (100%) .

Buffer

RSPO1 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

RSPO1 antibody can be stored at 4˚ C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Specificity

At least three isoforms of RSPO1 are known to exist; this antibody will detect the largest and smallest isoforms. RSPO1 antibody will not cross-react with other RSPO family members.

Isoform

Human RSPO1 has 3 isoforms, including isoform 1 (263aa, 29kD), isoform 2 (236aa, 26kD), and isoform 3 (200aa, 22kD) . Mouse RSPO1 had 1 isoform (265aa, 29kD) and rat RSPO1 had 1 isoform (262aa, 29kD) . 5171 can detect human, mouse and rat.

Symbol

RSPO1

Positive Control 2

Cat. No. 1301 - Human Heart Tissue Lysate

Positive Control 3

Cat. No. 10-501 - Human Heart Tissue Slide

Positive Control 4

Cat. No. 10-901 - Human Spleen Tissue Slide

NCBI Official Name

R-spondin homolog (Xenopus laevis)

NCBI Organism

Homo sapiens

Background Reference 01

Kamata et al. Biochim. Biophys. Acta 2004; 1676:51-61.

Background Reference 02

Kim et al. Cell Cycle 2006; 5:23-6.

Background Reference 03

Parma et al. Nat. Genet. 2006; 38:1304-9.

Background Reference 04

Chassot et al. Hum. Mol. Genet. 2008; 17:1278-91.

Other Product Names

RSPO1 Antibody: RSPO, CRISTIN3, R-spondin-1, Roof plate-specific spondin-1, hRspo1

Tested Applications

ELISA, WB, IHC-P, IF

Protein ID

84490388

Physical Properties

Liquid

Available Sizes

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

MBS7042271-01 0.02 mg

ATP synthase subunit b

Ask

View Details

MBS7042271-02 0.1 mg

ATP synthase subunit b

Ask

View Details

MBS7042271-03 5x 0.1 mg

ATP synthase subunit b

Ask

View Details

TA339711 100 µL

MED4 Rabbit Polyclonal Antibody

Ask

View Details

MBS7210034-01 48 Well

Canine Visual system homeobox 1 (VSX1) ELISA Kit

Ask

View Details

MBS7210034-02 96 Well

Canine Visual system homeobox 1 (VSX1) ELISA Kit

Ask

View Details