AP2M1 Antibody

AP2M1 Antibody: The heterotetrameric coat assembly protein complex, also known as the adaptor-related protein complex 2 (AP-2), belongs to the adaptor complexes medium subunits family. The mu 1 subunit of the AP-2 complex (AP2M1) is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. AP2M1 has also been shown to associate with the HIV-1 protein Nef, suggesting that Nef may use AP-2 complex to enhance the rate of endocytosis of both CD4 and class I MHC. AP2M1 may also play an important role in regulating the intracellular trafficking and function of cytotoxic T-lymphocyte associated (CTLA) -4 protein. At least two isoforms of AP2M1 are known to exist.

AP2M1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Aguilar RC, Ohno H, Roche KW, et al. Functional domain mapping of the clathrin-associated adaptor medium chains mu1 and mu J. Biol. Chem.1997; 272:27160-6.

Liu Q, Feng Y, and Forgac M. Activity and in vitro reassembly of the coated vesicle (H+) -ATPase requires the 50-kDa subunit of the clathrin assembly complex AP-2. J. Biol. Chem.1994; 269:31592-7.

Craig HM, Reddy TR, Riggs NL, et al. Interactions of HIV-1 Nef with the m subunits of adaptor protein complexes 1, 2, and 3: role of the dileucine-based sorting motif. Virology271:9-17.