CDC42 Antibody

Product Specifications

Background

CDC42 Antibody: CDC42 is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to S. cerevisiae CDC42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. CDC42 is thought to regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. At least two isoforms of CDC42 are known to exist.

NCBI Gene ID

998

Swiss Prot

P60953

Accession Number

NP_001782

Host

Chicken

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Homeostasis

Purification

CDC42 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1303 - Human Brain Tissue Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Pig: (100%), Chicken: (100%), Bovine: (100%), Guinea pig: (100%)

Buffer

CDC42 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

CDC42 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgY

Symbol

CDC42

NCBI Official Name

Cell division cycle 42 (GTP binding protein, 25kDa)

NCBI Organism

Homo sapiens

Background Reference 01

Erickson JW and Cerione RA. Multiple roles for Cdc42 in cell regulation. Curr. Opin. Cell Biol.2001; 13:153-7.

Background Reference 02

Chen W, Lim HH, and Lim L. The CDC42 homologue from Caenorhabditis elegans. Complementation of yeast mutation. J. Biol. Chem.1993; 268:13280-5.

Background Reference 03

Hart MJ, Eva A, Evans T, et al. Catalysis of guanine nucleotide exchange on the CDC42Hs protein by the dbl oncogene product. Nature1991; 354:311-4.

Background Reference 04

Rohatgi R, Ma L, Miki H, et al. The interaction between N-WASP and the Arp2/3 complex links Cdc42-dependent signals to actin assembly. Cell1999; 97:221-31.