TTBK2 Antibody

Product Specifications

Background

TTBK2 Antibody: Tau tubulin kinase (TTBK2) is a serine/threonine kinase whose substrates are thought to include the tau and tubulin proteins. Mutations in the TTBK2 gene cause spinocerebellar ataxia type 11 (SCA11), a neurodegenerative disease characterized by progressive ataxia and atrophy of the cerebellum and brainstem. Recent studies have shown that increased levels of TTBK2 expression in kidney carcinoma and melanoma cell lines increases resistance to the chemotherapeutic agent Sunitinib, and that reduction of TTBK2 though the use of siRNA sensitized these cell lines to the effects of Sunitib, suggesting that TTBK2 can be used as a resistance marker as well a potential target to overcome resistance.

NCBI Gene ID

146057

Swiss Prot

Q6IQ55

Accession Number

AAH71556

Host

Rabbit

Reactivity

Human

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Neuroscience

Purification

TTBK2 Antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Mouse: (88%)

Buffer

TTBK2 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

TTBK2 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Specificity

TTBK2 antibody is human specific. At least four isoforms of TTBK2 are known to exist; this antibody will detect only the two largest isoforms.

Symbol

TTBK2

NCBI Official Name

Tau tubulin kinase 2

NCBI Organism

Homo sapiens

Background Reference 01

Kitano-Takahashi M, Morita H, Kondo S, et al. Expression, purification and crystallization of a human tau-tubulin kinase 2 that phosphorylates tau protein. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 2007; 63:602-4.

Background Reference 02

Edener U, Kurth I, Meiner A, et al. Missense exchanges in the TTBK2 gene mutated in SCA11. J. Neurol. 2009; 1856-9.

Background Reference 03

Bender C and Ullrich A. PRKX, TTBK2 and RSK4 expression causes sunitinib resistance in kidney carcinoma- and melanoma cell lines. Int. J. Cancer 2011; epub.