Gle1 Antibody

Product Specifications

Background

Gle1 Antibody: The proper expression of gene products in eukaryotic cells relies on efficient transport of mRNA molecules out of the nucleus. Gle1 is an essential mRNA export factor in both human and yeast cells. It associates with the nuclear pore complex (NPC) through hCG1 and NUP155 in mammalian cells and in conjunction with inositol hexakisphosphate (IP6), stimulates Dbp5, a member of the DEAD-box helicase family, triggering mRNP remodeling and facilitating RNA export from the nucleus. Recent evidence suggests that mutations in Gle1 causing defects in mRNA export can result in human disease. At least three isoforms of Gle1 are known to exist.

NCBI Gene ID

2733

Swiss Prot

Q53GS7

Accession Number

NP_001003722

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Homeostasis

Purification

Gle1 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1403 - Mouse Brain Tissue Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Bovine: (100%)

Buffer

Gle1 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Gle1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Symbol

GLE1

NCBI Official Name

GLE1 RNA export mediator homolog (yeast)

NCBI Organism

Homo sapiens

Background Reference 01

Murphy R and Wente SR. An RNA-export mediator with an essential nuclear export signal. Nature1996; 383:357-60.

Background Reference 02

Watkins JL, Murphy R, Emtage JL, et al. The human homologue of Saccharomyces cerevisiae Gle1p is required for poly (A) + RNA export. Proc. Natl. Acad. Sci. USA1998; 95:6779-84.

Background Reference 03

Kendirgi F, Rexer DJ, Alcazar-Roman AR, et al. Interaction between the shuttling mRNA export factor Gle1 and the nucleoporin hCG1: A conserved mechanism in the export of Hsp70 mRNA. Mol. Biol. Cell2005; 16:4304-15.

Background Reference 04

Rayala HJ, Kendirgi F, Barry DM, et al. The mRNA export factor human Gle1 interacts with the nuclear pore complex protein Nup155. Mol. Cell. Proteomics2004; 3:145-55.