TOM70 Antibody

TOM70 Antibody: The translocase of outer mitochondrial membrane (TOM) complex is a multisubunit complex involved in the recognition, unfolding, and translocation of preproteins into the mitochondria. TOM70, an important member of the TOM complex, contains a tetratricopeptide repeat domain similar to those found in cytosolic chaperones such as Hsp90 and Hsc70 and provides a docking site for these proteins. This interaction is thought to be a critical first step in the TOM70-dependent mitochondrial import, followed by contact between the preprotein and TOM70. After targeting to TOM70, preproteins are translocated through the outer membrane via the TOM40 import pore complex. The precise mechanism of how preproteins progress from TOM70 to TOM40 to full translocation is still unclear. At least two isoforms of TOM70 are known to exist.

Independent Antibody Validation in Cell lines (Figure 1) shows similar TOM70 expression profile in human, mouse and rat cell lines detected by two independent anti-TOM70 antibodies that recognize different epitopes, 4949 against N-terminus domain and 4959 against central domain.TOM70 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines4949 4959

TOM70 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Human TOM70 has only one isoform (608aa, 67.5kD. Mouse TOM70 has one isoform (611aa, 67.6kD) and Rat TOM70 also has one isoform (610aa, 67.4kD) . 4959 can detect human, mouse and rat.