TOM70 Antibody

Product Specifications

Background

TOM70 Antibody: The translocase of outer mitochondrial membrane (TOM) complex is a multisubunit complex involved in the recognition, unfolding, and translocation of preproteins into the mitochondria. TOM70, an important member of the TOM complex, contains a tetratricopeptide repeat domain similar to those found in cytosolic chaperones such as Hsp90 and Hsc70 and provides a docking site for these proteins. This interaction is thought to be a critical first step in the TOM70-dependent mitochondrial import, followed by contact between the preprotein and TOM70. After targeting to TOM70, preproteins are translocated through the outer membrane via the TOM40 import pore complex. The precise mechanism of how preproteins progress from TOM70 to TOM40 to full translocation is still unclear.

NCBI Gene ID

9868

Swiss Prot

O94826

Accession Number

NP_055635

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

4949

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Validation

Independent Antibody Validation in Cell lines (Figure 1) shows similar TOM70 expression profile in human, mouse and rat cell lines detected by two independent anti-TOM70 antibodies that recognize different epitopes, 4949 against N-terminus domain and 4959 against central domain. TOM70 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines4949 4959

Field of Research

Homeostasis

Purification

TOM70 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1403 - Mouse Brain Tissue Lysate

Concentration

1 mg/mL

Buffer

TOM70 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

TOM70 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Specificity

At least two isoforms of TOM70 are known to exist; this antibody will only detect the larger isoform.

Isoform

Human TOM70 has only one isoform (608aa, 67.5kD. Mouse TOM70 has one isoform (611aa, 67.6kD) and Rat TOM70 also has one isoform (610aa, 67.4kD) . 4949 can detect human, mouse and rat.

Symbol

TOMM70A

Positive Control 2

Cat. No. 1202 - A431 lysate

Positive Control 3

Cat. No. 1224 - Daudi lysate

Positive Control 4

Cat. No. 1282 - 3T3/NIH lysate

NCBI Official Name

Translocase of outer mitochondrial membrane 70 homolog A (S. cerevisiae)

NCBI Organism

Homo sapiens

Background Reference 01

Kutik et al. J. Cell Biol. 2007; 179:585-91.

Background Reference 02

Young et al. Cell 2003; 112:41-50.

Other Product Names

TOM70 Antibody: KIAA0719, TOM70, Mitochondrial import receptor subunit TOM70, Mitochondrial precursor proteins import receptor

Tested Applications

ELISA, WB, IHC-P, IF

Protein ID

54607135

Physical Properties

Liquid

Available Sizes

Curated Selection

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