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PIG-Y Antibody

Product Specifications

Background

PIG-Y Antibody: Glycosylphosphatidylinositol (GPI) lipid anchoring is an important post-translational modification of proteins that takes place in the endoplasmic reticulum. The synthesis of GPI is initiated by GPI-N-acetylglucosaminyltransferase (GPI-GnT), a complex of proteins including PIG-A, PIG-H, PIG-C, GPI1, and DPM2. PIG-Y, the mammalian homolog to yeast Eri1p, is also thought to be involved in the biosynthesis of GPI. The PIG-Y gene encodes two proteins, one of which arises from leaky scanning of the mRNA.

NCBI Gene ID

84992

Swiss Prot

Q3MUY2

Accession Number

NP_116295

Host

Rabbit

Reactivity

Human, Mouse

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Homeostasis

Purification

PIG-Y Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1375 – Human Skeletal Muscle Tissue Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Bovine: (87%)

Buffer

PIG-Y Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

PIG-Y antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=4945

Fragment

IgG

Specificity

This antibody only detects isoform 1 of PIG-Y.

Symbol

PIGY

Positive Control 2

Cat. No. 10-901 - Human Spleen Tissue Slide

NCBI Official Name

Phosphatidylinositol glycan anchor biosynthesis, class Y

NCBI Organism

Homo sapiens

Background Reference 01

Eisenhaber B, Maurer-Stroh S, Novatchkova M, et al. Enzymes and auxiliary factors for GPI lipid anchor biosynthesis and post-translational transfer to proteins. Bioessays2003; 25:367-85.

Background Reference 02

Watanabe R, Murakami Y, Marmor MD, et al. Initial enzyme for glycosylphosphatidylinositol biosynthesis requires PIG-P and is regulated by DPM2. EMBO J.2000; 19:4402-11.

Background Reference 03

Murakami Y, Siripanyaphinoyo U, Hong Y, et al. The initial enzyme for glycosylphosphatidylinositol biosynthesis requires PIG-Y, a seventh component. Mol. Biol. Cell2005; 16:5236-46.

Other Product Names

PIG-Y Antibody: PIG-Y, Phosphatidylinositol N-acetylglucosaminyltransferase subunit Y, Phosphatidylinositol-glycan biosynthesis class Y protein, PIG-Y

Tested Applications

ELISA, WB, IHC-P, IF

Protein ID

14249680

Physical Properties

Liquid

Available Sizes

Curated Selection

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