Spred3 Antibody

Product Specifications

Background

Spred3 Antibody: Spred3 is a member of the Sprouty family, a group of proteins that act as negative regulators during development. Like Spred1 and Spred2, Spred3 contains and EVH1 domain in its amino terminus and a Sprouty-related cysteine-rich (SPR) domain in its C-terminus, but does not possess a functional c-kit binding domain (KBD) . The Spred proteins have also been implicated in the negative feedback regulation of FGF signaling in embryogenesis and angiogenesis. Although Spred3 was initially reported to be expressed in brain, at least one report also indicates that Spred3 can be found in liver. At least two isoforms of Spred3 are known to exist.

NCBI Gene ID

399473

Swiss Prot

Q2MJR0

Accession Number

NP_001035987

Host

Rabbit

Reactivity

Human

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Signal Transduction

Purification

Spred3 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1403 - Mouse Brain Tissue Lysate

Concentration

1 mg/mL

Buffer

Spred3 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Spred3 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Specificity

This Spred3 antibody is predicted to have no cross-reactivity to Spred1 or Spred2.

Symbol

SPRED3

Positive Control 2

Cat. No. 10-301 - Human Brain Tissue Slide

NCBI Official Name

Sprouty-related, EVH1 domain containing 3

NCBI Organism

Homo sapiens

Background Reference 01

Kato R, Nonami A, Taketomi T, et al. Molecular cloning of mammalian Spred-3 which suppresses tyrosine kinase-mediated Erk activation. Biochem. Biophys. Res. Commun.2003; 302:767-72.

Background Reference 02

Casci T, Vinos J, and Freeman M. Sprouty, an intracellular inhibitor of Ras signaling. Cell1999; 96:655-65.

Background Reference 03

Minowada G, Jarvis LA, Chi CL, et al. Vertebrate Sprouty genes are induced by FGF signaling and can cause chondrodysplasia when overexpressed. Development1999; 126:4465-75.