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Aak1 Antibody

Product Specifications

Background

Aak1 Antibody: AP2-associated protein kinase 1 (Aak1) is a member of the Ark1/Prk1 subfamily of Ser/Thr protein kinases that are thought to regulate endocytosis by phosphorylating the accessory endocytic components. Aak1 interacts with and phosphorylates the mu2 subunit of the AP-2 complex, which promotes binding of the AP-2 to tyrosine based (Yxxphi) internalization motif-containing receptors and subsequent receptor endocytosis. At least two isoforms of Aak1 are known to exist; the longer isoform contains an extended carboxy-terminus that contains an additional clathrin-binding domain. Overexpression of this long isoform or Aak1 depletion by RNA interference impairs transferrin recycling from the early/sorting endosome, suggesting that Aak1 functions at multiple steps of the endosomal pathway by regulating transferrin internalization and its recycling back to the plasma membrane.

NCBI Gene ID

22848

Swiss Prot

Q2M2I8

Accession Number

NP_055726

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Signal Transduction

Purification

Aak1 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1466 - Rat Spleen Tissue Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Pig: (100%), Bovine: (100%)

Buffer

Aak1 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Aak1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=4841

Fragment

IgG

Symbol

AAK1

Positive Control 2

Cat. No. 17-208 - A-20 Cell Slide

Positive Control 3

Cat. No. 10-301 - Human Brain Tissue Slide

NCBI Official Name

AP2 associated kinase 1

NCBI Organism

Homo sapiens

Background Reference 01

Connor SD and Schmid SL. Identification of an adaptor-associated kinase, AAK1, as a regulator of clathrin-mediated endocytosis. J. Cell Biol.2002; 156:921-9.

Background Reference 02

Smythe E and Ayscough KR. The Ark1/Prk1 family of protein kinases. Regulators of endocytosis and the actin skeleton. EMBO Rep.2003; 4:246-51.

Background Reference 03

Ricotta D, Connor SD, Schmid SL, et al. Phosphorylation of the AP2 m2 subunit by AAK1 mediates high affinity binding to membrane protein sorting signals. J. Cell Biol.2002; 156:791-5.

Background Reference 04

Connor SD and Henderson DM. A novel AAK1 splice variant functions at multiple steps of the endocytic pathway. Mol. Biol. Cell2007; 18:2698-706.

Other Product Names

Aak1 Antibody: KIAA1048, AP2-associated protein kinase 1, Adaptor-associated kinase 1

Tested Applications

ELISA, WB, ICC, IF

Protein ID

148277037

Physical Properties

Liquid

Available Sizes

Curated Selection

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