Integrin alpha 4 Antibody

Product Specifications

Background

Integrin alpha 4 Antibody: The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains. Alpha4 chain associates with either beta1 or beta7 chain. It has been demonstrated that the putative ligand-binding sites of both integrin alpha4beta1 and alpha4beta7 is located on the alpha4 chain. These ligands included Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin alpha4beta7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells.

NCBI Gene ID

3676

Swiss Prot

P13612

Accession Number

NP_000876

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Infectious Disease

Purification

Integrin alpha 4 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1466 - Rat Spleen Tissue Lysate

Concentration

1 mg/mL

Buffer

Integrin alpha 4 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Integrin alpha 4 antibody can be stored at 4˚ C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Specificity

At least two isoforms are known to exist. The immunogen to this antibody is within the Integrin alpha4 recombinant protein fragment and thus will recognize it.

Symbol

ITGA4

Positive Control 2

Cat. No. 17-005 - Jurkat Cell Slide

NCBI Official Name

Integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)

NCBI Organism

Homo sapiens

Background Reference 01

Wegener KL and Campbell ID. Transmembrane and cytoplasmic domains in integrin activation and protein-protein interactions. Mol. Membr. Biol.2008; 25:376-87.

Background Reference 02

Kamata T, Puzon W, and Takada Y. Identification of putative ligand-binding sites of the integrin alpha 4 beta 1 (VLA-4, CD49d/CD29) . Biochem J.1995; 305:945-51.

Background Reference 03

Berlin C, Berg EL, Briskin MJ, et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell1993; 74:185-95.

Background Reference 04

Pulido R, Elices MJ, Campanero MR, et al. Functional evidence for three distinct and independently inhibitable adhesion activities mediated by the human integrin VLA-4. Correlation with distinct alpha 4 epitopes. J. Biol. Chem.1991; 266:10241-5.