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GPAT1 Antibody

Product Specifications

Background

GPAT1 Antibody: Glycerol-3-phosphate acyltransferase 1 (GPAT1), one of four known GPAT isoforms, is located on the mitochondrial outer membrane, allowing reciprocal regulation with carnitine palmitoyltransferase-1. It is thought to be critical for the development of hepatic steatosis; steatosis triggered by GPAT1 overexpression leads to hepatic and possibly peripheral insulin resistance. GPAT1 is transcriptionally upregulated by insulin and sterol regulatory element binding protein (SREBP-1) and downregulated by AMP-activated protein kinase. Mice deficient in GPAT1 exhibit decreased triacylglycerol (TAG) in cardiomyocytes even in high-fat diets, suggesting that GPAT1 contributes significantly to TAG accumulation in heart tissue during lipogenic or high fat diets.

NCBI Gene ID

57678

Swiss Prot

Q9HCL2

Accession Number

NP_065969

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Obesity

Purification

GPAT1 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1463 - Rat Brain Tissue Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Pig: (100%), Bovine: (93%)

Buffer

GPAT1 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

GPAT1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=4613

Observed Molecular Weight

Lower molecular weight bands may represent alternate isoforms of GPAT1

Fragment

IgG

Specificity

At least four isoforms of GPAT1 are known to exist; this antibody will detect only the two largest isoforms.

Symbol

GPAM

NCBI Official Name

Glycerol-3-phosphate acyltransferase, mitochondrial

NCBI Organism

Homo sapiens

Background Reference 01

Coleman RA and Lee DP. Enzymes of triacylglycerol synthesis and their regulation. Prog. Lipid Res. 2004; 43:134-76.

Background Reference 02

Linden D, William-Olsson L, Ahnmark A, et al. Liver-directed overexpression of mitochondrial glycerol-3-phosphate acyltransferase results in hepatic steatosis, increased triacylglycerol secretion and reduced fatty acid oxidation. FASEB J. 2006; 20:434-43.

Background Reference 03

Eberle D, Hegarty B, Bossard P, et al. SREBP transcription factors: master regulators of lipid homeostasis. Biochimie 2004; 86:839-48.

Background Reference 04

Lewin TM, de Jong H, Schwerbrock NJ, et al. Mice deficient in glycerol-3-phosphate acyltransferase-1 have diminished myocardial triacylglycerol accumulation during lipogenic diet and altered phospholipid fatty acid composition. Biochim. Biophys. Acta 2008; 1781:352-8.

Other Product Names

GPAT1 Antibody: GPAT, GPAT1, KIAA1560, Glycerol-3-phosphate acyltransferase 1, mitochondrial, GPAT-1

Tested Applications

ELISA, WB, IHC-P, IF

Protein ID

190358539

Physical Properties

Liquid

Available Sizes

Curated Selection

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