VPS53 Antibody

Product Specifications

Background

VPS53 Antibody: The sorting of acid hydrolases to lysosomes rely on mannose 6-phosphate receptors that cycle between the trans-Golgi network (TGN) and endosomes. The maintenance of this cycle requires the function of the mammalian Golgi-associated retrograde protein (GARP) complex which is composed of three subunits: VPS52, VPS53, and VPS54. Depletion of any of these three proteins, such as by RNAi, impairs the retrograde transport of multiple TGN proteins. VPS53 was identified as an HIV dependency factor (HDF) and plays a role in viral entry to the cell, suggesting that VPS53 may be an important drug target in HIV treatment. At least five isoforms of VPS53 are known to exist.

NCBI Gene ID

55275

Swiss Prot

Q5VIR6

Accession Number

EAW90658

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Homeostasis

Purification

VPS53 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1210 - HEK293 Cell Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Chicken: (78%)

Buffer

VPS53 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

VPS53 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Symbol

VPS53

NCBI Official Name

Vacuolar protein sorting 53 homolog (S. cerevisiae)

NCBI Organism

Homo sapiens

Background Reference 01

Pfeffer SR. Targeting of proteins to the lysosome. Curr. Top. Microbiol. Immunol.1991; 170:43-65.

Background Reference 02

Liewen H, Meinhold-Heerlein I, Oliveira V, et al. Characterization of the human GARP (Golgi associated retrograde protein) complex. Exp. Cell Res.2005; 306:22-34.

Background Reference 03

Perez-Victoria FJ, Mardones GA, and Bonifacino JS. Requirement of the human GARP complex for mannose 6-phosphate-receptor-dependent sorting of cathepsin D to lysosomes. Mol. Biol. Cell2008; 19:2350-62.

Background Reference 04

Brass AL, Dykxhoorn DM, Benita Y, et al. Identification of host proteins required for HIV infection through a functional genomic screen. Science2008; 319:921-6.