CXCR4-Lo Antibody

Product Specifications

Background

CXCR4-Lo Antibody: Human immunodeficiency virus (HIV) and related viruses require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. Among them, CXCR4 is a principal coreceptor for T-cell tropic strains of HIV-1 fusion and entry of human white blood cells. CXCR4 is also required for the infection by dual-tropic strains of HIV-1 and mediates CD-4 independent infection by HIV-2. The alpha-chemokine SDF-1 is the ligand for CXCR4 and prevents infection by T-tropic HIV-1. CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells. CXCR4 messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV binding sites.

NCBI Gene ID

7852

Swiss Prot

P61073

Accession Number

NP_001008540

Host

Rabbit

Reactivity

Human

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Infectious Disease

Purification

CXCR4-Lo Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1306 - Human Spleen Tissue Lysate

Concentration

1 mg/mL

Buffer

CXCR4-Lo Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

CXCR4-Lo antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Specificity

This antibody is specific for the longer isoform of CXCR4.

Symbol

CXCR4

Positive Control 2

Cat. No. 17-001 - HeLa Cell Slide

NCBI Official Name

Chemokine (C-X-C motif) receptor 4

NCBI Organism

Homo sapiens

Background Reference 01

Dimitrov DS. Cell1997; 91:721-30.

Background Reference 02

Feng Y et al. Science1996; 272:872-7.

Background Reference 03

Berson JF et al. J. Virol.1996; 70:6288-95.

Background Reference 04

Doranz BJ et al. Cell 1996; 85:1149-58.

Other Product Names

CXCR4-Lo Antibody: FB22, HM89, LAP3, LCR1, NPYR, WHIM, CD184, LESTR, NPY3R, NPYRL, HSY3RR, NPYY3R, D2S201E, C-X-C chemokine receptor type 4, FB22, CXC-R4