Nhe-1 Antibody

Product Specifications

Background

Nhe-1 Antibody: The Na+/H+ antiporter (Nhe-1) is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells and is specifically inhibited by the diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, and tumor promoters. Nhe-1 acts as an anchor for actin filaments to control the integrity of the cortical cytoskeleton. This occurs through a previously unrecognized structural link between Nhe-1 and the actin-binding proteins ezrin, radixin, and moesin, collectively referred to as ERM proteins. A structural role for Nhe-1 has been proposed in regulating the cortical cytoskeleton that is independent of its function as an ion exchanger. It is also thought that Nhe-1 play a role in hypertension.

NCBI Gene ID

6548

Swiss Prot

P19634

Accession Number

P19634

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Neuroscience

Purification

Nhe-1 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1465 - Rat Kidney Tissue Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Rabbit: (95%), Pig: (95%), Bovine: (95%)

Buffer

Nhe-1 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Nhe-1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Specificity

At least three isoforms of Nhe-1 are known to exist.

Symbol

SLC9A1

Positive Control 2

Cat. No. 10-301 - Human Brain Tissue Slide

NCBI Official Name

Solute carrier family 9 (sodium/hydrogen exchanger), member 1

NCBI Organism

Homo sapiens

Background Reference 01

Mendoza SA. The Na+-H+ antiport is a mediator of cell proliferation. Acta Paediatr. Scand. 1987; 76:545-7.

Background Reference 02

Denker SP, Huang DC, Orlowski J, et al. Direct binding of the NA—H exchanger NHE1 to ERM proteins regulates the cortical cytoskeleton and cell shape independently of H (+) translocation. Mol. Cell. 2000; 6:1425-36.

Background Reference 03

Cingolani HE, Rebolledo OR, Portiansky EL, et al. Regression of hypertensive myocardial fibrosis by NA (+) /H (+) exchange inhibition. Hypertension 2003; 41:373-7.