MANF Antibody

MANF Antibody: MANF, also known as ARMET, was initially identified as a protein containing an arginine-rich region that was highly mutated in a variety of tumors. More recently it was identified as a mesencephalic astrocyte-derived neurotrophic factor with selectivity for dopaminergic neurons, similar to glial cell line-derived neurotrophic factor (GDNF) and CDNF. In rat brain slices, MANF enhanced nigral gamma-aminobutyric acid release. Like GDNF and CDNF, MANF has selective neuroprotective activity for dopaminergic neurons suggesting that it may be indicated for the treatment of Parkinson's disease. Expression of MANF has also been shown to be induced during ER stress, suggesting that it may play a role in protein quality control during ER stress.

KO Validation (Figure 1) : Anti-MANF antibodies (4347) specificity was further verified by MANF specific knockout. MANF signal was not detected in MANF knockout HEK293T cells as compared to that in control wild type cells. KO Validation4347) Recombinant Protein Test (Figure 3) : Anti-MANF antibodies (4347) detected human MANF recombinant protein at different concentrations. Recombinant Protein Test4347 Localization Validation (Figure 8) : MANF detected by anti-MANF antibodies is colocalized with HuC/D (a general neural marker) . MANF is localized in mature neuronal cells. Localization Validation KD Validation (Figure 9) : Anti-MANF antibody (4347) specificity was further verified by MANF specific knockdown. MANF signal, when injected with a combination of two antisense splice-blocking morpholino oligonucleotides (MOsp), was disrupted in comparison with either wild type or when injected with control MO . KD Validation4347) Induced Expression Validation (Figure 10) : MANF expression detected by anit-MANF antibodies was up-regulated by VPA treatment. Induced Expression Validation

MANF antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Human MANF has one isoform (182aa, 21kD) . Mouse MANF has one isoform (179aa, 20kD) and Rat MANF also has one isoform (179aa, 20kD) . 4347 can detect human, mouse and rat.