TRIM25 Antibody

Product Specifications

Background

TRIM25 Antibody: TRIM25, also known as estrogen responsive finger protein (EFP) is a member of the RING finger-B box-coiled coil family and is a downstream target of estrogen receptor a. Mice lacking this gene displayed an underdeveloped uterus and reduced estrogen responsiveness demonstrating that this protein is essential for estrogen dependent proliferation. TRIM25 expression is increased in breast carcinoma where it promotes the growth of breast tumor by functioning as an E3 ubiquitin ligase that targets the negative cell cycle checkpoint 14-3-3s. Recent reports have indicated that TRIM25 E3 ubiquitin ligase is essential for the retinoic-acid-inducible gene 1 (RIG-1) signaling pathway to elicit host antiviral innate immunity.

NCBI Gene ID

7706

Swiss Prot

Q14258

Accession Number

NP_005073

Host

Rabbit

Reactivity

Human

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Homeostasis

Purification

TRIM25 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1201 - HeLa Cell Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Mouse: (80%)

Buffer

TRIM25 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

TRIM25 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Symbol

TRIM25

Positive Control 2

Cat. No. 17-001 - HeLa Cell Slide

NCBI Official Name

Tripartite motif-containing 25

NCBI Organism

Homo sapiens

Background Reference 01

Inoue S, Orimo A, Hosoi T, et al. Genomic binding-site cloning reveals an estrogen-responsive gene that encodes a RING finger protein. Proc. Natl. Acad. Sci. USA1993; 11117-20.

Background Reference 02

Orimo A, Inoue S, Minowa O, et al. Underdeveloped uterus and reduced estrogen responsiveness in mice with disruption of the estrogen-responsive finger protein gene, which is a direct target of estrogen receptor a. Proc. Natl. Acad. Sci. USA1999; 96:12027-32.

Background Reference 03

Thomson SD, Ali S, Pickles L, et al. Analysis of estrogen-responsive finger protein expression in benign and malignant human breast. Int. J. Cancer2001; 91:152-8.