ORAI1 Antibody

Product Specifications

Background

ORAI1 Antibody: Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI1 is a recently identified four-transmembrane spanning protein that is an essential component of CRAC. A missense mutation in this protein in humans is the cause of one form of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry. It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor.

NCBI Gene ID

84876

Swiss Prot

Q96D31

Accession Number

Q96D31

Host

Rabbit

Reactivity

Human, Mouse

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Immunology

Purification

ORAI1 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1316 - Human Ovary Tissue Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Rat: (93%), Chicken: (100%)

Buffer

ORAI1 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

ORAI1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Specificity

This antibody is predicted to have no cross-reactivity to ORAI2 or ORAI3.

Symbol

ORAI1

Positive Control 2

Cat. No. 11-201 - Human Ovary Tissue Slide

Positive Control 3

Cat. No. 10-901 - Human Spleen Tissue Slide

NCBI Official Name

ORAI calcium release-activated calcium modulator 1

NCBI Organism

Homo sapiens

Background Reference 01

Lewis RS. Calcium signaling mechanisms in T lymphocytes. Annu. Rev. Immunol.2001; 19:497-521.

Background Reference 02

Feske S, Gwack Y, Prakriya M, et al. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature2006; 441:179-85.

Background Reference 03

Soboloff J, Spassova MA, Dziadek MA, et al. Calcium signals mediated by STIM and Orai proteins - a new paradigm in inter-organelle communication. Biochim. Biophys. Acta.2006; 1763:1161-8.