Bub1 Antibody

Product Specifications

Background

Bub1 Antibody: Bub1 was initially identified as the mammalian homolog to the yeast protein through a cDNA screen. Bub1 encodes a kinase involved in the formation of the spindle checkpoint, an important mechanism that ensures high fidelity mitotic chromosome segregation. It is thought to be required for assembly of a functional inner centromere, sister chromatid cohesion via targeting of the Shugoshim protein and metaphase congression. Bub1 functions by phosphorylating cdc20, a member of the mitotic checkpoint complex and activating the spindle checkpoint. A related protein kinase Bub3 interacts with Bub1 and targets it to kinetochores prior to chromosome alignment. Mutations in bub1 have been associated with aneuploidy and several forms of cancer.

NCBI Gene ID

699

Swiss Prot

O43683

Accession Number

O43683

Host

Rabbit

Reactivity

Human, Mouse

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Homeostasis, Signal Transduction, Cancer

Purification

Bub1 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1288 - A20 Cell Lysate

Concentration

1 mg/mL

Buffer

Bub1 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Bub1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Symbol

BUB1

Positive Control 2

Cat. No. 17-208 - A-20 Cell Slide

NCBI Official Name

Budding uninhibited by benzimidazoles 1 homolog (yeast)

NCBI Organism

Homo sapiens

Background Reference 01

Pangilinan F, Li Q, Weaver T, et al. Mammalian BUB1 protein kinases: map positions and in vivo expression. Genomics1997; 46:379-88.

Background Reference 02

Williams GL, Roberts TM and Gjoerup OV. Bub1: Escapades in a cellular world. Cell Cycle2007; 6:1699-704.

Background Reference 03

Meraldi P and Sorger PK. A dual role for Bub1 in the spindle checkpoint and chromosome congression. EMBO J.2005; 24:1621-33.

Background Reference 04

Tang Z, Shu H, Oncel D, et al. Phosphorylation of cdc20 by bub1 provides a catalytic mechanism for APC/C inhibition by the spindle checkpoint. Mol. Cell2004; 16:387-97.