PD-1 Antibody

Product Specifications

Background

PD-1 Antibody: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC) . T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases.

NCBI Gene ID

5133

Swiss Prot

Q15116

Accession Number

Q15116

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

4065

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Validation

KO Validation (Figure 1) : Anti-PD-1 antibodies (4065) specificity was further verified by PD-1 specific knockout. PD-1 signal was not detected in PD-1 knockout HeLa cells as compared to that in control wild type cells. KO Validation4065) KD validation (Figure 2) : Anti-PD-1 antibody (4065) specificity was further verified by PD-1 specific knockdown. PD-1 signal in HeLa cells transfected with PD-1 siRNAs or shRNA was disrupted in comparison with that in cells transfected with control siRNAs. KD validation4065

Field of Research

Apoptosis

Purification

PD-1 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1204 - K562 Cell Lysate

Concentration

1 mg/mL

Buffer

PD-1 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

PD-1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

Human PD-1 has 1 isoform (288aa, 32kD) . Mouse PD-1 has 1 isoform 288aa, 32kD) and Rat PD-1 also has one isoform (287aa, 32kD) . 4065 can detect human, mouse and rat.

Fragment

IgG

Isoform

Human PD-1 has 1 isoform (288aa, 32kD) . Mouse PD-1 has 1 isoform 288aa, 32kD) and Rat PD-1 also has one isoform (287aa, 32kD) . 4065 can detect human, mouse and rat.

Symbol

PDCD1

Positive Control 2

Cat. No. 1205 - Jurkat Cell Lysate

Positive Control 3

Cat. No. 1208 - THP-1 Cell Lysate

Positive Control 4

Cat. No. 10-301 - Human Brain Tissue Slide

NCBI Official Name

Programmed cell death 1

NCBI Organism

Homo sapiens

Background Reference 01

Holling et al. Hum. Immunol. 2004; 65:282-90.

Background Reference 02

Ishida et al. EMBOJ. 1992;11:3887-95.

Background Reference 03

Zhong et al. Int. Immunol. 2004; 16:1181-8

Background Reference 04

Nishimura et al. Immunity 1999;11:141-51

Other Product Names

PD-1 Antibody: PD1, PD-1, CD279, SLEB2, hPD-1, hPD-l, hSLE1, PD1, Programmed cell death protein 1, Protein PD-1, PDCD1, PDCD-1

Tested Applications

ELISA, WB, IHC-P, IF

Protein ID

145559515

Physical Properties

Liquid

Available Sizes

Curated Selection

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