PD-L1 Antibody

Product Specifications

Background

PD-L1 plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/CD279, PD-L1 modulates the activation threshold of T-cells and limits T-cell effector response (1) . The PDCD1/CD279-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (2,3) . Through a yet unknown activating receptor, it may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (4) .

NCBI Gene ID

29126

Swiss Prot

Q9NZQ7

Accession Number

NP_054862

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

4059

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Validation

Independent Antibody Validation (Figure 2) shows similar PD-L1 expression profile in both human and mouse cell lines detected by two independent anti-PD-L1 antibodies that recognize different epitopes, 4059 against the center of human PD-L1 and RF16035 against the extracellular domain. PD-L1 proteins are detected in most of the cell lines, but not in A549 and THP-1 cells by the two independent antibodies. Independent Antibody Validation4059 RF16035 siRNA Knockdown Validation (Figure 3) : Anti-PD-L1 antibody (4059) specificity was further verified by PD-L1 specific siRNA knockdown. PD-L1 signal in HeLa cells transfected with PD-L1 siRNAs was much weaker in comparison with that in HeLa cells transfected with control siRNAs. siRNA Knockdown Validation4059 Overexpression Validation (Figure 4) : Anti-PD-L1 antibodies (4059) can detect the overexpression of PD-L1 protein in 293 cells transfected with PD-L1. Overexpression Validation4059

Field of Research

Apoptosis

Purification

PD-L1 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1201 - HeLa Cell Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Rat: (77%), Mouse: (71%)

Buffer

PD-L1 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

PD-L1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Specificity

PD-L1 antibody has no cross-reactivity to PD-L2.

Isoform

Human PD-L1 has 3 isoforms, including isoform 1 (290aa, 33.3kD), isoform 2 (176aa, 20.2kD), and isoform 3 (178aa, 20.5kD) . This antibody detects human isoform 1&3, mouse and rat PD-L1 (290aa, 33kD for both of them) .

Symbol

CD274

Positive Control 2

Cat. No. 1224 - Daudi Cell Lysate

Positive Control 3

Cat. No. 1204 - K562 Cell Lysate

Positive Control 4

Cat. No. 10-501 - Human Heart Tissue Slide

Positive Control 5

Cat. No. 1301 - Human Heart Tissue Lysate

NCBI Official Name

CD274 molecule

NCBI Organism

Homo sapiens

Background Reference 01

Freeman et al. Exp. Med. 2000; 192:1027-34.

Background Reference 02

Burr et al. Nature 2017; 549:101-5.

Background Reference 03

Mezzadra et al. Nature 2017; 549:106-10.

Background Reference 04

Dong et al. Nat. Med. 1999 5:1365-9.

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Other Product Names

PD-L1 Antibody: B7-H, B7H1, PDL1, PD-L1, PDCD1L1, PDCD1LG1, Programmed cell death 1 ligand 1, B7 homolog 1

Tested Applications

ELISA, WB, IHC-P, IF, Flow

Protein ID

7661534

Physical Properties

Liquid

Available Sizes

Curated Selection

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