BANF1 Antibody

Product Specifications

Background

BANF1 Antibody: Barrier-to-autointegration factor 1 (BANF1) is a conserved chromatin protein that non-specifically binds double-stranded DNA. BANF1 also interacts with a family of nuclear proteins that include LAP2, emerin, and MAN1. It is also a host cell component of retroviral pre-integration complexes (PICs), including that of HIV. BANF1 will bind to p55 Gag (the structural precursor of HIV-1 virions) as well as its cleaved product matrix. In addition to being a host cell component of the PIC, it is thought that BANF1 is also present at low levels in incoming virions, and thus might contribute to the assembly or activity of HIV-1 PICs through direct binding to matrix as well as DNA.

NCBI Gene ID

8815

Swiss Prot

O75531

Accession Number

AAH05942

Host

Rabbit

Reactivity

Human

Immunogen

4017

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Validation

Independent Antibody Validation in Cell lines (Figure 1) shows similar BANF1 expression profile in human and mouse cell lines detected by two independent anti-BANF1 antibodies that recognize different epitopes, 4017 against C-terminus domain and 4019 against N-terminus domain. BANF1 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines4017 4019

Field of Research

Infectious Disease

Purification

BANF1 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1305 - Human Kidney Tissue Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Mouse: (100%), Rat: (100%)

Buffer

BANF1 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

BANF1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Isoform

Human BANF1 has one isoform (89aa, 10kD) . Mouse BANF1 has one isoform (89aa, 10kD) and Rat BANF1 also has one isoform (89aa, 10kD) . 4017 can detect human, mouse and rat.

Symbol

BANF1

Positive Control 2

Cat. No. 10-401 - Human Kidney Tissue Slide

NCBI Official Name

Barrier to autointegration factor 1

NCBI Organism

Homo sapiens

Background Reference 01

Furukawa. J. Cell Sci. 1999; 112:2485-92.

Background Reference 02

Cai et al. EMBO J. 2001; 20:4399-407.

Background Reference 03

Chen and Engelman. Proc. Natl. Acad. Sci. USA 1998; 95:15270-4.

Other Product Names

BANF1 Antibody: BAF, NGPS, BCRP1, D14S1460, BAF, BCRG1, Barrier-to-autointegration factor, Breakpoint cluster region protein 1

Tested Applications

ELISA, WB, IF

Protein ID

13543577

Physical Properties

Liquid

Available Sizes

Curated Selection

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