FAF1 Antibody

FAF1 Antibody: Fas-associated protein 1 (FAF1) was initially identified as a Fas-binding pro-apoptotic protein that is component of the death-inducing signaling complex in Fas-mediated apoptosis. FAF1 can also induce apoptosis in the absence of extrinsic death signals when overexpressed although it does not contain typical death motifs such as the death domain, death effector domain, and caspase recruitment domain. Overexpression of FAF1 also decreases the basal level of NF-κ B activity in transfected 293 cells, inhibits NF-κ B activity induced by TNF-α , IL-1β and lipopolysaccharide, and prevents NF-κ B translocation to the nucleus, suggesting that another role of FAF1 is to negatively regulate the activity of NF-κ B. FAF1 can also interact with the inflammatory signaling PYRIN-containing Apaf-1-like proteins (PYPAFs, also called NALPs) such as PYPAF1, PYPAF2 (NALP2), and PYPAF7, suggesting FAF1 may also be involved in the inflammation pathway. Multiple differentially spliced isoforms of FAF1 are known to exist.

FAF1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Ryu SW and Kim E. Apoptosis induced by human Fas-associated factor 1, hFAF1, requires its ubiquitin homologous domain, but not the Fas-binding domain. Biochem. Biophys. Res. Commun. 2001; 286:1027-32.

Park M-Y, Jang HD, Lee SY, et al. Fas-associated Factor-1 inhibits Nuclear Factor-κ B (NF-κ B) activity by interfering with nuclear translocation of the RelA (p65) subunit of NF-κ B. J. Biol. Chem.2004; 279:2544-9.

Kinoshita T, Kondoh C, Hasegawa M, et al. Fas-associated factor 1 is a negative regulator of PYRIN-containing Apaf-1-like protein 1. Int. Immunol. 2006;18:1701-6.