RIG-1 Antibody

Product Specifications

Background

RIG-1 Antibody: The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses. Like the toll-like receptor 3 (TLR3), the cytoplasmic helicase retinoic acid inducible gene protein 1 (RIG-1) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. Unlike TLR3 however, RIG-1 activates the kinases TBK1 and IKKε through the adaptor protein IPS-1. These kinases then phosphorylate the transcription factors IRF-3 and IRF-7 which are essential for the expression of type-I interferons. RIG-1 is required for the production of interferons in response to RNA viruses including paramyxoviruses, influenza virus, and Japanese encephalitis virus.

NCBI Gene ID

23586

Swiss Prot

O95786

Accession Number

O95786

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

RIG-1 antibody was raised against human GST-tagged RIG-1 protein.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Infectious Disease

Purification

RIG-1 Antibody is Protein A purified.

Positive Control

Cat. No. 1285 - C2C12 Cell Lysate

Concentration

1 mg/mL

Buffer

RIG-1 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

RIG-1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Symbol

DDX58

Positive Control 2

Cat. No. 1301 - Human Heart Tissue Lysate

Positive Control 3

Cat. No. 10-501 - Human Heart Tissue Slide

NCBI Official Name

DEAD (Asp-Glu-Ala-Asp) box polypeptide 58

NCBI Organism

Homo sapiens

Background Reference 01

Akira S, Uematsu S, and Takeuchi O. Pathogen recognition and innate immunity. Cell 2006; 124:783-801.

Background Reference 02

Yoneyama M, Kikuchi M, Natsukawa T, et al. The RNA helicase RIG-I has an essential function in double-stranded RNA-induced innate antiviral responses. Nat. Immunol. 2004; 5:730-7.

Background Reference 03

Alexopoulou L, Holt AC, Medzhitov R, et al. Recognition of double-stranded RNA and activation of NF-kappaB by Toll-like receptor 3. Nature 2001; 413:732-8.