PKR Antibody

Product Specifications

Background

PKR Antibody: The interferon-inducible, double-stranded RNA (dsRNA) -dependent protein kinase PKR is a member of the eukaryotic initiation factor-2 alpha (eIF2-alpha) kinase family, possessing serine-threonine kinase activity and two dsRNA-binding motifs that acts as part of the innate immune system. Upon binding dsRNA, PKR undergoes a conformational change leading to its activation and its phosphorylation of the translation factor eIF2, resulting in a general shutdown of protein synthesis and induction of apoptosis through upregulation of caspase-8 and capsase-9 activity in order to prevent the production of more viruses. To evade the antiviral effects of PKR, viruses have evolved multiple mechanisms, such as the inhibition of PKR by the non-structural protein (NS1) of the influenza virus. More recently, PKR has been implicated in several neurodegenerative diseases including Alzheimer, Huntington, and amyotrophic lateral sclerosis.

NCBI Gene ID

5610

Swiss Prot

P19525

Accession Number

AAP57628

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

3947

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Validation

Independent Antibody Validation in Cell lines (Figure 2) shows similar PKR expression profile in human and mouse cell lines detected by two independent anti-PKR antibodies that recognize different epitopes, 3947 against C-terminus domain and 3949 against N-terminus domain. PKR proteins are detected in all the tested human cell lines except SK-N-SH cell line at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines3947

Field of Research

Innate Immunity

Purification

PKR Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1202 - A431 Cell Lysate

Concentration

1 mg/mL

Buffer

PKR Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

PKR antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Specificity

At least two different isoforms of PKR are known to exist; this antibody will detect both isoforms.

Isoform

Human PKR has 2 isoforms, including isoform 1 (551aa, 62.1kD) and isoform S (510aa, 57.4kD. This antibody detects both human isoforms. Mouse PKR has 1 isoform (515aa, 58.3kD) . Rat PKR has only one isoform identified so far (513aa, 58.3kD) .

Symbol

EIF2AK2

Positive Control 2

Cat. No. 1462 - Rat Lung Tissue Lysate

Positive Control 3

Cat. No. 1208 - THP-1 cell lysate

Positive Control 4

Cat. No. 1224 - Daudi Cell Lysate

NCBI Official Name

Eukaryotic translation initiation factor 2-alpha kinase 2

NCBI Organism

Homo sapiens

Background Reference 01

Meurs et al. Cell 1990; 62:379-90.

Background Reference 02

Saelens et al. J. Biol. Chem. 2001; 276:41620-8.

Background Reference 03

Gil and Esteban. Apoptosis 2000; 5:107-14.

Background Reference 04

Gil et al. FEBS Lett. 2002; 19:3665-74.

Other Product Names

PKR Antibody: PKR, PRKR, EIF2AK1, PKR, Interferon-induced, double-stranded RNA-activated protein kinase, Eukaryotic translation initiation factor 2-alpha kinase 2, eIF-2A protein kinase 2