ATM Antibody

Product Specifications

Background

ATM Antibody: DNA double strand breaks represent a major threat to an organism's genome. Eukaryotic cells have developed mechanisms that sense the presence this damage and initiate suitable responses that can include DNA repair, cell cycle delay, and programmed cell death. The ATM (mutated in Ataxia-Telangiectasia) protein kinase is activated following the formation of DNA double strand breaks, phosphorylating p53 and another kinase CHK2. This initiates a signaling cascade leading to the phosphorylation and inhibition of Cdc25, ultimately preventing cell cycle progression. In some cell types, such as the hemapoietic system, this leads to apoptosis instead of cell cycle arrest. Multiple isoforms of ATM are known to exist.

NCBI Gene ID

472

Swiss Prot

Q13315

Accession Number

AAB65827

Host

Rabbit

Reactivity

Human

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Homeostasis, Cancer

Purification

ATM Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1224 - Daudi Cell Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Pig: (86%), Mouse: (71%)

Buffer

ATM Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

ATM antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Symbol

ATM

Positive Control 2

Cat. No. 1369 - Human Lymph node Tissue Lysate

Positive Control 3

Cat. No. 11-521 - Human Lymphoid Tissue Tissue Slide

NCBI Official Name

Ataxia telangiectasia mutated

NCBI Organism

Homo sapiens

Background Reference 01

Cahill D, Connor B, and Carney JP. Mechanisms of eukaryotic DNA double strand break repair. Front. Biosci. 2006 ; 11 :1958-76.

Background Reference 02

Dasika GK, Lis SC, Zhao S, et al. DNA damage-induced cell cycle checkpoints and DNA strand break repair in development and tumorigenesis. Oncogene 1999; 18:7883-99.