TANK Antibody

TANK Antibody: TANK was initially identified as a novel TRAF-interacting protein that regulated TRAF-mediated signal transduction. Specifically, ligand binding by surface receptors in the tumor necrosis factor (TNF) receptor and Toll/interleukin-1 (IL-1) receptor families lead to the formation of a TRAF/TANK complex that mediates the activation of the transcription factor NF-κ B. This activation of NF-κ B occurs through an association with the kinases IKKε and TBK1. More recently, it was shown that these proteins can then form a complex with NEMO, a protein that regulates the activity of the Iκ B complex. This suggests that in addition to the possibility that TBK1 and IKKε activate the IKKs, the association with the IKK complex may help these kinases modulate other functions, such as the transactivation potential of NF-κ B proteins. At least two isoforms of TANK are known to exist.

TANK antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Rothe M, Xiong J, Shu HB, et al. I-TRAF is a novel TRAF-interacting protein that regulates TRAF-mediated signal transduction. Proc. Natl. Acad. Sci. USA 1996; 93:8241-6.

Pomerantz JL and Baltimore D. NF-κ B activation by a signaling complex containing TRAF2, TANK and TBK1, a novel IKK-related kinase. EMBO J. 1999; 18:6694-704.

Chariot A, Leonardi A, Muller J, et al. Association of the adaptor TANK with the Iκ B kinase (IKK) regulator NEMO connects IKK complexes with the IKKε and TBK1 kinases. J. Biol. Chem.2002; 277:37029-36