Bfl-1 Antibody

Product Specifications

Background

Bfl-1 Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by caspase activation. Proteins that comprise the Bcl-2 family appear to control the activation of these enzymes. One such member is multi-domain antiapoptotic protein Bfl-1, which is overexpressed in stomach and other cancers. Bfl-1 can interact with Bax and suppress apoptosis by inhibiting the release of cytochrome c and caspase-3 activation. It is upregulated in cisplatin-resistant human bladder tumors, suggesting that its expression may be important for cisplatin resistance and inhibition of apoptosis in cancer cells. At least two isoforms of Bfl-1 are known to exist.

NCBI Gene ID

597

Swiss Prot

Q16548

Accession Number

NP_004040

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Apoptosis, Neuroscience

Purification

Bfl-1 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1305 - Human Kidney Tissue Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Bovine: (87%)

Buffer

Bfl-1 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Bfl-1 antibody can be stored at 4˚ C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Specificity

At least two isoforms of Bfl-1 are known to exist; this antibody will detect both isoforms.

Symbol

BCL2A1

NCBI Official Name

BCL2-related protein A1

NCBI Organism

Homo sapiens

Background Reference 01

Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.

Background Reference 02

Choi SS, Park IC, Yun JW, et al. A novel Bcl-2 related gene, Bfl-1, is overexpressed in stomach cancer and preferentially expressed in bone marrow. Oncogene 1995; 11:1693-8.

Background Reference 03

Kim JK, Kim KD, Lee E, et al. Up-regulation of Bfl-1/A1 via NF-κ B activation in cisplatin-resistant human bladder cancer cell line. Cancer Lett. 2004; 212:61-70.

Background Reference 04

Zhang H, Cowan-Jacob SW, Simonen M, et al. Structural basis of BFL-1 for its interaction with BAX and its anti-apoptotic action in mammalian and yeast cells. J. Biol. Chem.2000; 275:11092-9.