Bfl-1 Antibody

Product Specifications

Background

Bfl-1 Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by caspase activation. Proteins that comprise the Bcl-2 family appear to control the activation of these enzymes. One such member is multi-domain antiapoptotic protein Bfl-1, which is overexpressed in stomach and other cancers. Bfl-1 can interact with Bax and suppress apoptosis by inhibiting the release of cytochrome c and caspase-3 activation. It is upregulated in cisplatin-resistant human bladder tumors, suggesting that its expression may be important for cisplatin resistance and inhibition of apoptosis in cancer cells.

NCBI Gene ID

597

Swiss Prot

Q16548

Accession Number

NP_004040

Host

Rabbit

Reactivity

Human, Mouse

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Apoptosis

Purification

Bfl-1 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1305 - Human Kidney Tissue Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Bovine: (92%)

Buffer

Bfl-1 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Bfl-1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Specificity

At least two isoforms of Bfl-1 are known to exist; this antibody will only detect the larger isoform.

Symbol

BCL2A1

Positive Control 2

Cat. No. 1405 - Mouse Kidney Tissue Lysate

Positive Control 3

Cat. No. 1302 - Human Lung Tissue Lysate

NCBI Official Name

BCL2-related protein A1

NCBI Organism

Homo sapiens

Background Reference 01

Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.

Background Reference 02

Choi SS, Park IC, Yun JW, et al. A novel Bcl-2 related gene, Bfl-1, is overexpressed in stomach cancer and preferentially expressed in bone marrow. Oncogene 1995; 11:1693-8.

Background Reference 03

Kim JK, Kim KD, Lee E, et al. Up-regulation of Bfl-1/A1 via NF-κ B activation in cisplatin-resistant human bladder cancer cell line. Cancer Lett. 2004; 212:61-70.

Background Reference 04

Zhang H, Cowan-Jacob SW, Simonen M, et al. Structural basis of BFL-1 for its interaction with BAX and its anti-apoptotic action in mammalian and yeast cells. J. Biol. Chem. 2000; 275:11092-9.