Cyclin O Antibody

Product Specifications

Background

Cyclin O Antibody: Cyclin O, also known as CCNO, has recently been identified as a Cdk1- and Cdk2-activating cyclin specific to apoptosis in lymphoid cells. Cyclin O binds to and activates Cdk2 in response to instrinsic apoptotic stimuli such as glucocorticoids or DNA-damaging agents. Supression of Cyclin O expression by shRNA leads to the inhibition of glucocorticoid and DNA-damage-induced apoptosis due to a failure of apical caspase activation while leaving the CD95 death receptor-mediated apoptosis intact. Note: this gene, which had a previous symbol of UNG2, was erroneously identified as a uracil DNA glycosylase. A later publication identified this gene's product as a cyclin protein family member.

NCBI Gene ID

10309

Swiss Prot

P22674

Accession Number

NP_066970

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Apoptosis

Purification

Cyclin O Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1410 - Mouse Bladder Tissue Lysate

Concentration

1 mg/mL

Buffer

Cyclin O Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Cyclin O antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

39 kDa

Fragment

IgG

Specificity

At least two isoforms of Cyclin O are known to exist; this antibody will recognize both isoforms.

Symbol

CCNO

NCBI Official Name

Cyclin O

NCBI Organism

Homo sapiens

Background Reference 01

Roig MB, Roset R, Ortet L, et al. Identification of a novel cyclin required for the intrinsic apoptosis pathway in lymphoid cells. Cell Death Diff. 2009; 16:230-43.