Bik Antibody
Product Specifications
Background
Bik Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by the activation of proteolytic enzymes termed caspases. Proteins that comprise the Bcl-2 family appear to control the activation of these enzymes. One such protein BIK was recently identified as an endoplasmic reticulum (ER) -residing pro-apoptotic member of the Bcl-2 homology domain-3 (BH3) -only group of the Bcl-2 family that stimulates mitochondrial release of cytochrome c following p53 induction of apoptosis. A significant fraction of BIK is found as an ER transmembrane protein, with most of the protein facing the cytosol. Restricting BIK to the ER membrane by replacing the transmembrane region with that of the ER-selective membrane anchor of cytochrome b resulted in a decreased cytochrome c release from mitochondria and a corresponding drop in cell death. Recent evidence suggests that BIK cooperates with NOXA, another BH3-only protein, to somehow enhance the activation of Bax to stimulate the rapid release of cytochrome c from mitochondria.
NCBI Gene ID
638
Swiss Prot
Q13323
Accession Number
CAG30276
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Apoptosis, Innate Immunity
Purification
Bik Antibody is affinity chromatography purified via peptide column.
Positive Control
Cat. No. 1205 - Jurkat Cell Lysate
Concentration
1 mg/mL
Buffer
Bik Antibody is supplied in PBS containing 0.02% sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Bik antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=3819
Fragment
IgG
Symbol
BIK
Positive Control 2
Cat. No. 17-005 - Jurkat Cell Slide
NCBI Official Name
BCL2-interacting killer (apoptosis-inducing)
NCBI Organism
Homo sapiens
Background Reference 01
Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.
Background Reference 02
Germain M, Mathai JP, and Shore GC. BH-3-only BIK functions at the endoplasmic reticulum to stimulate cytochrome c release from mitochondria. J. Biol. Chem. 277:18053-60.
Background Reference 03
Germain M, Mathai JP, McBride HM, et al. Endoplasmic reticulum BIK initiates DRP1-regulated remodelling of mitochondrial cristae during apoptosis. EMBO J. 2005; 24:1546-56.
Other Product Names
Bik Antibody: BP4, NBK, BIP1, Bcl-2-interacting killer, Apoptosis inducer NBK
Tested Applications
ELISA, WB, ICC, IF
Protein ID
47678311
Physical Properties
Liquid
Available Sizes
Curated Selection
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