TIM-4 Antibody

Product Specifications

Background

TIM-4 Antibody: The T cell immunoglobulin and mucin domain containing protein (TIM) family encodes cell surface receptors that are involved in the regulation of T helper (Th) -1 and -2 cell-mediated immunity. TIM-4, which is preferentially expressed on macrophages and dendritic cells, is the natural ligand of TIM-1, and this binding leads to T-cell expansion and cytokine production. Unlike other members of the TIM family, TIM-4 lacks a putative tyrosine phosphorylation signal sequence in its intracellular domain. The TIM-4 gene maps to a locus associated with predisposition to asthma in both mice and humans and with its connection to TIM-1-triggered Th2 responsiveness, may be considered as a candidate disease/predisposition gene for asthma.

NCBI Gene ID

91937

Swiss Prot

Q96H15

Accession Number

NP_612388

Host

Rabbit

Reactivity

Human, Mouse

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Immunology, Growth Factors, Signal Transduction

Purification

TIM-4 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1205 - Jurkat Cell Lysate

Concentration

1 mg/mL

Buffer

TIM-4 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

TIM-4 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

Observed: 45 kDa

Fragment

IgG

Specificity

At least four isoforms of TIM-4 are known to exist; this antibody will detect all four isoforms.

Symbol

TIMD4

Positive Control 2

Cat. No. 1283 - RAW 264.7 Cell Lysate

Positive Control 3

Cat. No. 17-005 - Jurkat Cell Slide

NCBI Official Name

T-cell immunoglobulin and mucin domain containing 4

NCBI Organism

Homo sapiens

Background Reference 01

Meyers JH, Sabatos CA, Chakravarti S, et al. The TIM family regulates autoimmune and allergic diseases. Trends Mol. Med. 2005; 11:362-9.

Background Reference 02

Meyers JH, Chakravarti S, Schlesinger D, et al. TIM-4 is the ligand for TIM-1, and the TIM-1-TIM4 interaction regulates T cell proliferation. Nat. Immunol. 2005; 6:455-64.

Background Reference 03

Kuchroo VK, Umetsu DT, DeKruyff RH, et al. The TIM gene family: emerging roles in immunity and disease. Nat. Rev. Immunol. 2003; 3:454-62.

Background Reference 04

Shakhov AN, Rybtsov S, Tumanov AV, et al. SMUCKLER/TIM4 is a distinct member of TIM family expressed by stromal cells of secondary lymphoid tissues and associated with lymphotoxin signaling.