TIM-1 Antibody

Product Specifications

Background

TIM-1 Antibody: The human form of TIM-1 was initially discovered as a membrane glycoprotein through which the hepatitis A virus can gain entry into a cell. It was also identified as kidney injury molecule 1 (Kim-1), a predicted adhesion molecule that is upregulated on the surfaces of kidney epithelia. It is also expressed on T helper 2 (Th2) cells of the immune system, and following the binding of its natural ligand TIM-4, stimulates T cell expansion and cytokine production in response to viral challenge. It has been suggested that hyperactivation of TIM-1 leads to an increased level of Th2 responsiveness and asthma susceptibility, and antibodies to TIM-1 may therefore be a novel approach to treating asthma.

NCBI Gene ID

26762

Swiss Prot

Q96D42

Accession Number

NP_036338

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

3809

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Validation

Independent Antibody Validation in Cell lines (Figure 2) shows similar TIM-1 expression profile in human cell lines detected by two independent anti-TIM-1 antibodies that recognize different epitopes, 3809 against N-terminus domain and 3811 against the central domain. TIM-1 proteins are detected in all the tested cell lines except MCF-7 at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines3809 3811 siRNA knockdown validation (Figure 3) : Anti-TIM-1 antibody (3809) specificity was further verified by TIM-1 specific siRNA knockdown. TIM-1 signal in HeLa cells transfected with TIM-1 siRNAs was much weaker in comparison with that in HeLa cells transfected with control siRNAs. siRNA knockdown validation3809

Field of Research

Immunology, Neuroscience, Signal Transduction, Growth Factors

Purification

TIM-1 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1202 - A431 Cell Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Rat: (56.3%)

Buffer

TIM-1 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

TIM-1 antibody can be stored at 4˚ C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Isoform

Human TIM-1 has 5 isoforms, including isoform a precursor (364aa, 39kD), isoform a (364aa, 39kD), isoform b precursor (401aa, 44kD), isoform b (359aa, 39kD), and isoform c (359aa, 39kD) . This antibody detects human isoform b precursor. Mouse TIM-1 has 3 isoforms, including isoform a precursor (305aa, 33kD), isoform b precursor (282aa, 31kD) and isoform b (282aa, 31kD) . Rat TIM-1 has only one isoform identified so far (307aa, 34kD) .

Symbol

HAVCR1

Positive Control 2

Cat. No. 1203 - A549 Cell Lysate

Positive Control 3

Cat. No. 1224 - Daudi Cell Lysate

Positive Control 4

Cat. No. 11-401 - Human Uterus Tissue Slide

NCBI Official Name

Hepatitis A virus cellular receptor 1

NCBI Organism

Homo sapiens

Background Reference 01

Feigelstock et al. J. Virol. 1998; 72:6621-8

Background Reference 02

Ichimura et al. J. Biol. Chem. 1998; 273:4135-42

Background Reference 03

Meyers et al. Mol. Med. 2005; 11:362-9

Background Reference 04

Mayers et al. Nat. Immunol. 2005;6:455-64.

Other Product Names

TIM-1 Antibody: TIM, KIM1, TIM1, HAVCR, KIM-1, TIM-1, TIMD1, TIMD-1, HAVCR-1, Hepatitis A virus cellular receptor 1, Kidney injury molecule 1, HAVcr-1

Tested Applications

ELISA, WB, IHC-P, IF

Protein ID

153085427

Physical Properties

Liquid

Available Sizes

Curated Selection

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