IL-27 Antibody

Product Specifications

Background

IL-27 Antibody: Like interleukin-23 (IL-23), IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the p40-related protein EBI3 and an IL-12 p35-related protein termed p28. IL-27 is produced after activation by antigen-presenting cells and induces proliferation of naïve but not memory CD4+ T-cells. It acts by binding to its receptor WSX-1 and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting the IL-27 is involved in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-gamma, and T-bet.

NCBI Gene ID

246778

Swiss Prot

Q8NEV9

Accession Number

NP_663634

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

3799

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Validation

Independent Antibody Validation in Cell lines (Figure 2) shows similar IL-27 expression profile in human and mouse cell lines detected by two independent anti-IL-27 antibodies that recognize different epitopes, 3797 against N-terminus domain and 3799 against central domain. IL-27 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines3797 3799 Recombinant Protein Test (Figure 3) shows that anti-IL-27 antibody (3799) detects human recombinant IL-17 (IL-17A + IL-17B) protein that contains the amino acids of 3799 antigenic peptide. Recombinant Protein Test

Field of Research

Immunology, Cancer

Purification

IL-27 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1288 - A20 Cell Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Pig: (92%), Rat: (79%)

Buffer

IL-27 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

IL-27 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Isoform

Human IL-27 has one isoform (243aa, 28kD) . Mouse IL-27 has one isoform (234aa, 27kD) and rat IL-27 also has one isoform (234aa, 27kD) . 3797 can detect human, mouse and rat IL-27.

Symbol

IL27

Positive Control 2

Cat. No. 17-207 - EL4 Cell Slide

Positive Control 3

Cat. No. 1201 - HeLa Cell Lysate

Positive Control 4

Cat. No. 1205 - Jurkat Cell Lysate

Positive Control 5

Cat. No. 1220 - SK-N-SH Cell Lysate

NCBI Official Name

Interleukin 27

NCBI Organism

Homo sapiens

Background Reference 01

Pfanz et al. Immunity 2002; 16:779-90.

Background Reference 02

Pfanz et al. J. Immunol. 2004; 172:2225-31.

Background Reference 03

Hisada et al. Cancer Res. 2004; 64:1152-6.

Other Product Names

IL-27 Antibody: p28, IL30, IL-27, IL27A, IL-27A, IL27p28, Interleukin-27 subunit alpha, p28, IL-27 subunit alpha

Tested Applications

ELISA, WB

Protein ID

28416913

Physical Properties

Liquid

Available Sizes

Curated Selection

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